Identification of Reed-Berezovsky-Sternberg cells in lymphatic nodes in classic Hodgkin's lymphoma by flow cytometry: a clinical case series
نویسندگان
چکیده
Despite their B cell origin, Reed-Berezovsky-Sternberg tumor cells (RBS) in classic Hodgkin's lymphoma (cHL) demonstrate an absolutely unique phenotype. Immunohistochemistry of RBS is positive for CD15 antigen most cases, CD30, PAX-5; they do not express the T CD3, В CD19, and cases are negative CD20, as well common leukocyte CD45. Taking into account such unequivocal immunophenotype, can be identified by multiparameter flow cytometry. Thus, J.R. Fromm et al. (2006, 2014) have convincingly shown possibility to identify a puncture and/or biopsy sample lymphatic nodes cHL were fair opinion that rather simple reproducible technique cytometry could additional diagnostic instrument cHL.
 We tested proposed assessment node involvement used 8 10-parameter detection samples node, confirmed feasibility high performance also performed morphological immunohistochemical from patients with suspected cHL. The study included clinical immunohistochemically (n = 8), control those other diagnoses than lymphoma. In all we found cells. future plan analyze larger case
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ژورنال
عنوان ژورنال: Al?manah Klini?eskoj Mediciny
سال: 2023
ISSN: ['2587-9294', '2072-0505']
DOI: https://doi.org/10.18786/2072-0505-2023-51-015